A Simple Pipetting-based Method for Encapsulating Live Cells into Multi-layered Hydrogel Droplets
| Autor: | Changyoon Baek, Sung Hee Chung, Ju Hun Yeon, Hyundoo Hwang, Junhong Min |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Alternative methods Chemistry HEK 293 cells technology industry and agriculture Biomedical Engineering Pipette Substrate (chemistry) Bioengineering 02 engineering and technology 021001 nanoscience & nanotechnology complex mixtures Embryonic stem cell 03 medical and health sciences 030104 developmental biology In vivo Cancer cell Biophysics Electrical and Electronic Engineering 0210 nano-technology Biotechnology |
| Zdroj: | BioChip Journal. 12:184-192 |
| ISSN: | 2092-7843 1976-0280 |
| Popis: | Here, we present a simple pipetting-based approach for generating multi-layered core-shell hydrogel droplets composed of alginate for outer shell and collagen for culturing cells inside. By using a multi-hole plastic substrate and by pipetting, multi-layered hydrogel droplets were generated in a simple and rapid manner. HEK293 cells, which are human embryonic kidney cells, were cultured for 14 days in double-layered hydrogel droplet with high viability. Cancer cells were co-cultured with epithelial cells in multi-layered hydrogel droplets and applied for drug tests with curcumin. As epithelial cells protect cancer cells from anti-cancer drugs, co-cultured cells showed lower sensitivity to curcumin. We developed a simple and easy method for creating complex hydrogel particles for 3D multicellular co-culture and developed an alternative method for drug testing in vivo. |
| Databáze: | OpenAIRE |
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