Anchoring a cytoactive factor in a wound bed promotes healing
| Autor: | Kathleen M. Guthrie, Leandro B. C. Teixeira, Jonathan F. McAnulty, Sayani Chattopadhyay, Christopher J. Murphy, Ronald T. Raines, Richard R. Dubielzig |
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| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty integumentary system Chemistry Biomedical Engineering Medicine (miscellaneous) Anchoring Substance P Pharmacology Surgery Biomaterials Extracellular matrix 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology medicine Wound healing |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 10:1012-1020 |
| ISSN: | 1932-6254 |
| DOI: | 10.1002/term.1886 |
| Popis: | Wound healing is a complex process that requires the intervention of cytoactive factors. The one-time application of soluble factors to a wound bed does not maintain a steady, sufficient concentration. Here we investigated the benefits of anchoring a factor in a wound bed via a tether to endogenous collagen. We used a collagen-mimetic peptide (CMP) as a pylon. The CMP binds to damaged but not intact collagen and thus localizes a pendant cytoactive factor in the regions of a wound bed that require intervention. As a model factor, we chose substance P, a peptide of the tachykinin family that promotes wound healing. Using splinted wounds in db/db mice, we found that the one-time application of a CMP-substance P conjugate enhances wound healing compared to unconjugated substance P and other controls. Specifically, all 16 wounds treated with the conjugate closed more thoroughly and, did so with extensive re-epithelialization and mitigated inflammatory activity. These data validate a simple and general strategy for re-engineering wound beds by the integration of beneficial cytoactive factors. Copyright © 2014 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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