Semiquantitative Analysis of Apolipoprotein A-I Modified by Advanced Glycation End Products in Diabetes Mellitus
| Autor: | Shinichi Munekata, Tatsumi Moriya, Zensuke Ogawa, Tohru Akahoshi, Yoshifumi Kurosaki, Tomoaki Tsukushi |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty Apolipoprotein B biology Biochemistry (medical) Clinical Biochemistry Methylglyoxal Public Health Environmental and Occupational Health Case-control study Hematology medicine.disease Medical Laboratory Technology chemistry.chemical_compound Insulin resistance Endocrinology chemistry Glycation Internal medicine Diabetes mellitus medicine biology.protein Immunology and Allergy lipids (amino acids peptides and proteins) Glycated hemoglobin Lipoprotein |
| Zdroj: | Journal of Clinical Laboratory Analysis. 27:231-236 |
| ISSN: | 0887-8013 |
| Popis: | Background Apolipoprotein A-I (Apo A-I), the major component of high-density lipoprotein (HDL), is modified by reactive α-oxoaldehydes, such as methylglyoxal (MG) and glycolaldehyde (GA), and these modifications affect the function of Apo A-I. GA- and MG-modified Apo A-I serum levels were semiquantitatively evaluated in diabetic patients to elucidate the association of each protein with diabetes and to determine its appropriateness as a serum marker of diabetes. Methods We enrolled 44 subjects in this study (diabetic subjects, n = 24; nondiabetic subjects, n = 20). GA- and MG-modified Apo A-I levels in serum were determined by sandwich enzyme-linked immunosorbent assay (ELISA) by using anti-GA or anti-MG antibody and anti-Apo A-I antibody. Results The GA-modified Apo A-I levels did not significantly differ between the diabetic and nondiabetic subjects (1.00 ± 0.38 vs. 0.96 ± 0.22). However, the MG-modified Apo A-I levels in the diabetic subjects were significantly higher than those in the nondiabetic subjects (1.33 ± 0.52 vs. 0.90 ± 0.20). In addition, MG-modified Apo A-I levels correlated with the glycated hemoglobin (HbA1c) levels, HDL-cholesterol levels, and the homeostasis model assessments of insulin resistance, which are indicators of insulin resistance. Conclusion The MG-modified Apo A-I level may be an indicator of diabetic dyslipidemia and insulin resistance. |
| Databáze: | OpenAIRE |
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