Transgenic Rice Plants After Direct Gene Transfer into Protoplasts
| Autor: | Kokichi Hinata, Youichi Arimoto, Kinya Toriyama, Hirofumi Uchimiya |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Genetics
Oryza sativa Electroporation fungi Genetic transfer Biomedical Engineering food and beverages Bioengineering Chimeric gene Protoplast Biology Applied Microbiology and Biotechnology Genetically modified rice chemistry.chemical_compound chemistry Biochemistry Molecular Medicine Gene DNA Biotechnology |
| Zdroj: | Nature Biotechnology. 6:1072-1074 |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/nbt0988-1072 |
| Popis: | We have regenerated whole plants of rice (Oryza sativa L.) derived from protoplasts, which had been electroporated with plasmid DNA possessing a chimeric gene encoding aminoglycoside phosphotransferase II (APH(3′)II). Transformed calli were selected on the basis of tolerance to the antibiotic, G418. Several plants were regenerated possessing functional APH(3′)II activity due to the integration of intact foreign DNA into their genome. |
| Databáze: | OpenAIRE |
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