Sucrose, cytokinin, and ethylene influence formation of in vitro bulblets in onion and leek

Autor: E. R. J. Keller
Rok vydání: 1993
Předmět:
Zdroj: Genetic Resources and Crop Evolution. 40:113-120
ISSN: 1573-5109
0925-9864
Popis: In vitro maintenance of plant organs can enhance programs in plant breeding and germplasm resources. In bulbous plants, such as onion (Allium cepa L.) and leek (A. ampeloprasum L.) induction and storage of in vitro bulblets could enable long term maintenance of special genotypes. In vitro cultivated seedlings of onion and leek were induced to form bulblets by increase in sucrose concentration (30, 50, 150 g/1), and addition of benzyladenine (BA-0, 12.5 mg/1), or ethephon (0, 5, 20 days). The highest bulbing ratios were obtained within combinations of sucrose and ethephon treatments. BA caused not only bulb swelling but also an increase of multiple adventitious shoot formation. Increasing the sucrose concentration and treatment with ethephon are used to obtain bulblets for in vitro storage under conditions of slow growth.
Databáze: OpenAIRE