Biochip for genotyping SNPs defining core Y-chromosome haplogroups in Russian population groups

Autor:	Zasedatelev Alexander, Fesenko Denis, Nasedkina Tatyana, Surzhikov Sergey, Chudinov Alexander
Rok vydání:	2014
Předmět:	Genetics Biomedical Engineering Bioengineering Single-nucleotide polymorphism Biology Y chromosome Molecular biology Haplogroup Russian population SNP Electrical and Electronic Engineering Allele Biochip Genotyping Biotechnology
Zdroj:	BioChip Journal. 8:171-178
ISSN:	2092-7843 1976-0280
DOI:	10.1007/s13206-014-8303-z
Popis:	A biochip Ycore10 has been developed which allows identification of the following SNP markers of Y haplogroups: M130 (C), M145 (DE), P257 (G), M69(H), U179 (I), M304 (J), M185 (L), M231 (N), M175 (O) and P224 (R). The biochip can be used in determination of paternal ethnicity of offenders in the complex ethnic milieu of Russia. The sensitivity limit of the biochip-based assay was 31 pg of DNA template. Ycore10 genotyping was successfully performed with a range of male DNA concentrations (0.5–300 ng). Altogether 89 samples were genotyped and the following haplogroups were detected: C (2), DE (10), G (6), H (2), I (20), J (6), L (1), N (17), O (2), R (22). In one sample the ancestral alleles were detected for all ten biallelic markers. This tool is feasible for genotyping of mixed and degraded samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d8298f4e85a898324239f669737647e4 https://doi.org/10.1007/s13206-014-8303-z Zobrazit plný text záznamu Full text from SpringerLink