| Autor: |
Ingrid Fritsch, Timothy J. Muldoon, Amy J. Powless, Foysal Z. Khan, Courtney J Hunter, Joshua A. Hutcheson, Devin Benson |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management. |
| ISSN: |
0277-786X |
| Popis: |
We present a light sheet confocal microscope (LSCM) capable of high-resolution imaging of cell suspensions in a microfluidic environment. In lieu of conventional pressure-driven flow or mechanical translation of the samples, we have employed a novel method of fluid transport, redox-magnetohydrodynamics (redox-MHD). This method achieves fluid motion by inducing a small current into the suspension in the presence of a magnetic field via electrodes patterned onto a silicon chip. This on-chip transportation requires no moving parts, and is coupled to the remainder of the imaging system. The microscopy system comprises a 450 nm diode 20 mW laser coupled to a single mode fiber and a cylindrical lens that converges the light sheet into the back aperture of a 10x, 0.3 NA objective lens in an epi-illumination configuration. The emission pathway contains a 150 mm tube lens that focuses the light onto the linear sensor at the conjugate image plane. The linear sensor (ELiiXA+ 8k/4k) has three lateral binning modes which enables variable detection aperture widths between 5, 10, or 20 μm, which can be used to vary axial resolution. We have demonstrated redox-MHD-enabled light sheet microscopy in suspension of fluorescent polystyrene beads. This approach has potential as a high-throughput image cytometer with myriad cellular diagnostic applications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
