MORPHOFUNCTIONAL STATE OF HEPATOCYTES UNDER THE EXPOSURE TO SODIUM FLUORIDE
| Autor: | I.Yu. Bagmut, I. L. Kolisnyk |
|---|---|
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії. 20:196-199 |
| ISSN: | 2077-1126 2077-1096 |
| DOI: | 10.31718/2077-1096.20.3.196 |
| Popis: | The article provides the data on the morphofunctional state of hepatocytes in the liver of white rats under the subtoxic action of sodium fluoride. Mature rats of the Wistar population (N = 17), weighing 180-210 g, were intragastrically injected with aqueous solutions of sodium fluoride in a dose of 1/10 LD50 at the ratio of 20 mg / kg of animal body weight daily. The subacute experiment lasted 60 days. Studying hepatocytes in the rat liver and assessing their morphological rearrangement at the subcellular level of organization was carried out by electron microscopy. The study of ultrastructural organization of the liver under the influence of sodium fluoride revealed changes in the submicroscopic architecture characteristic of the development of dystrophic processes. Prolonged intoxication with sodium fluoride caused a number of changes in the liver ultrastructurer, manifested by the development of intracellular edema in hepatocytes, swelling of mitochondria, changes in the density of their matrix, partial reduction and loss of cristae, vacuolization and expansion of the cisterns of the granular endoplasmic reticulum, an increase in the number of primary lysosomes, redistribution chromatin of the nucleus and a decrease in the number of ribosomes and glycogen granules. These changes indicate a disruption of bioenergetics of hepatocytes associated with the mitochondrial apparatus and the development of hypoxic processes, which lead to a decrease in the activity of redox reactions occurring at the level of intracellular membranes and organelles. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|