LC–ESI–MS/MS study of carvedilol and its stress degradation products

Autor:	C. Purna Chander, B. Raju, Ragampeta Srinivas, Asmath Sulthana
Rok vydání:	2013
Předmět:	Chromatography Chemistry General Chemical Engineering General Engineering Mass spectrometry Analytical Chemistry Solvent Stress (mechanics) Hydrolysis chemistry.chemical_compound medicine Degradation (geology) Acetonitrile Carvedilol Ammonium acetate medicine.drug
Zdroj:	Analytical Methods. 5:4330
ISSN:	1759-9679 1759-9660
Popis:	An isocratic liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI–MS) method has been developed for the separation and identification of stress degradation products (DPs) of carvedilol (CAR), a non-selective β-blocker with vasodilatory properties. CAR was subjected to hydrolytic (acidic, alkaline and neutral), oxidative, photolytic and thermal stress conditions as per ICH guidelines Q1A (R2). The drug degraded under acidic, basic and photolytic stress conditions while it was stable under neutral, thermal and oxidative stress conditions. A total of five DPs were formed which were all well resolved from the parent drug as well as from each other on an Agilent XDB C-18 column (4.6 × 150 mm, 5 μm) using a mobile phase comprising of 20 mM ammonium acetate (pH adjusted to 6) (solvent A) and acetonitrile (solvent B) in an isocratic elution method. The structures of the degradation products were elucidated by using LC–ESI–MS/MS combined with accurate mass measurements.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d81fe988d4c42ccf90ce8f5afd3b1091 https://doi.org/10.1039/c3ay40804c Zobrazit plný text záznamu