Prevalence of latent virus in Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) and its activation by a heterologous virus

Autor:	Lucien N’Guessan Kouassi, Kanetosi Kusigemati, Yositaka Sakamaki, Katsuo Tsuda, Masayuki Nakamura, Shigeyuki Mukawa, Chie Goto
Rok vydání:	2009
Předmět:	Lepidoptera genitalia Mythimna separata Latent Virus biology Insect Science fungi Spodoptera litura Noctuidae Virulence biology.organism_classification Nested polymerase chain reaction Virology Virus
Zdroj:	Applied Entomology and Zoology. 44:95-102
ISSN:	1347-605X 0003-6862
DOI:	10.1303/aez.2009.95
Popis:	Nested PCR amplification of a Spodoptera litura nucleopolyhedrovirus (SpltNPV) lef-8 gene fragment was performed on egg masses and larvae of S. litura, and resulted in detection of a latent SpltNPV in 20.0% of “healthy” laboratory stock samples, and in 22.6% of samples collected at different periods and in remote places in Kagoshima Prefecture. The PCR product sequences showed 99% similarity to the published lef-8 sequence of SpltNPV, confirming that the amplification products were derived from SpltNPV. Further, cross-inoculation of laboratory stock S. litura larvae with Mythimna separata NPV (MyseNPV) activated the latent virus, which provoked a change in the virulence of MyseNPV samples collected from cadavers of S. litura. Our data suggest that caution should be taken when using S. litura as a host to produce heterologous NPVs.
Databáze:	OpenAIRE
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