Mechanism of disinfection

Autor: Leela Iyengar, A. V. S. Prabhakara Rao, C. Venkobachar
Rok vydání: 1975
Předmět:
Zdroj: Water Research. 9:119-124
ISSN: 0043-1354
DOI: 10.1016/0043-1354(75)90160-8
Popis: Certain biochemical aspects of mechanism of disinfection are discussed in the paper. The total dehydrogenase activity of intact E. coli cells is affected by chlorine dose and this correlates with per cent survivals. The succinic dehydrogenase activity in the crude extract of E. coli is affected by chlorine within the bactericidal concentrations. The dose required for inhibition of above enzyme is less than that for intact cells. To deliniate the mechanism of inhibition of succinic dehydrogenase, the effect of chlorine on thiol groups present in crude extract is studied. After chlorine exposure there is a reduction in thiol groups indicating the thiol groups to be vulnerable to the oxidising influence of chlorine. The inhibition of succinic dehydrogenase induced due to lower doses of chlorine can significantly be reversed with reduced glutathione. The ATP-ase which is localised in cytoplasmic membrane of cell, like succinic dehydrogenase, is not affected to any degree by chlorine in bactericidal concentrations. The catalase activity of E. coli is not affected.
Databáze: OpenAIRE