Bone Marrow Erythroid Precursor Ca++ Regulates the Response to Human Recombinant Erythropoietin (Rhuepo) in Hemodialysis Patients
| Autor: | Caviglia Pm, Ramello A, Schelotto C, Salit M, Silvia Carozzi, Cantaluppi A, M. G. Nasini, Silvano Lamperi |
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| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
030232 urology & nephrology Biomedical Engineering Medicine (miscellaneous) chemistry.chemical_element Bioengineering 030204 cardiovascular system & hematology Calcium law.invention Biomaterials 03 medical and health sciences 0302 clinical medicine In vivo law hemic and lymphatic diseases Internal medicine medicine Erythroid Precursor Cells General Medicine In vitro medicine.anatomical_structure Endocrinology chemistry Erythropoietin Recombinant DNA Erythropoiesis Bone marrow medicine.drug |
| Zdroj: | The International Journal of Artificial Organs. 13:747-750 |
| ISSN: | 1724-6040 0391-3988 |
| DOI: | 10.1177/039139889001301107 |
| Popis: | The modulation of erythropoiesis by erythropoietin is conditioned by the concentration of Ca++ in the cytoplasm of the bone marrow erythroid precursors. We evaluated in vitro erythroid colony development from bone marrow erythroid precursors incubated with increasing concentrations of Ca++ or Ca++ plus 1,25 (OH)2 D3, and bone marrow erythroid precursor cytoplasmic Ca++ concentrations in 10 anemic hemodialysis (HD) patients before and during rHuEPO therapy. Results showed that: a) in vitro: in uremics patients before rHuEPO therapy, bone marrow erythroid precursor cytoplasmic Ca++ was lower than in normal subjects; the addition of Ca++ to the bone marrow erythroid precursors induced a dose-dependent Ca++ and erythroid colony development increase; 1,25 (OH)2 D3 potentiated this effect; b) in vivo: rHuEPO normalized bone marrow erythroid precursor Ca++ and erythroid colony development. An inverse correlation was seen between bone marrow erythroid colony development, precursor CA++ before therapy, the in vitro erythroid and the rHuEPO dose needed in vivo to normalize hematological parameters. These data emphasize the role of Ca++ in erythropoiesis and may aid understanding of the mode of action of rHuEPO in HD. |
| Databáze: | OpenAIRE |
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