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Background: Since the prostate is located in an area that is susceptible to infectious agents in urine, it is rational to hypothesize that infectious agent that causes inflammation may be important in causing prostate cancer. To date, there is no single microorganism that is recognized to aid prostate cancer aetiology, yet various species of bacteria are doubted or known to induce prostatitis, symptomatic bacterial prostatitis-related microbes and pro-inflammatory bacterium. Setting and Design: The left-over urine samples were collected from patients diagnosed with carcinoma of the prostate attending the Urologic Clinic with a prior consent form after a histopathological diagnosis was performed. Materials and Methods: The urine samples were streaked over petri plates (MacConkey & Blood agar), Antibiotic susceptibility was conducted using the Kirby-Bauer disc diffusion method following DNA extraction, PCR and 16s rRNA sequencing of the resistant isolates. Statistical Analysis: IBM SPSS version 20.0 was used for mean and standard error calculations along with percentages. Results: Urine samples showed the presence of polymicrobial isolates (85%) with Escherichia coli being the predominant organism (31.00%). Among 48 isolates, 23 were found resistant to antibiotic treatment. The sequencing analysis revealed that Escherichia coli (7), Klebsiella pneumoniae (7) were predominant followed by Enterococcus faecalis (5), Proteus vulgaris (2) and Staphylococcus aureus (1), Pseudomonas aeruginosa (1). Conclusions: The urine analysis revealed polymicrobial isolates indicating the presence of high microbial flora in men diagnosed with prostate cancer, indicating the prevalence of uropathogens and pro-inflammatory bacteria in the urinary tract of prostate cancer patients. |
