Nanospectrofluorometry inside single living cell by scanning near-field optical microscopy

Autor:	J. F. Angiboust, Michel Troyon, M. Spajer, Franck H. Lei, M. Manfait, H. Morjani, G. Y. Shang
Rok vydání:	2001
Předmět:	Materials science Physics and Astronomy (miscellaneous) business.industry Confocal Shear force Near and far field Laser Fluorescence Fluorescence spectroscopy law.invention Optics Optical microscope law Near-field scanning optical microscope business
Zdroj:	Applied Physics Letters. 79:2489-2491
ISSN:	1077-3118 0003-6951
DOI:	10.1063/1.1409586
Popis:	Near-field fluorescence spectra with subdiffraction limit spatial resolution have been taken in the proximity of mitochondrial membrane inside breast adenocarcinoma cells (MCF7) treated with the fluorescent dye (JC-1) by using a scanning near-field optical microscope coupled with a confocal laser microspectrofluorometer. The probe–sample distance control is based on a piezoelectric bimorph shear force sensor having a static spring constant k=5 μN/nm and a quality factor Q=40 in a physiological medium of viscosity η=1.0 cp. The sensitivity of the force sensor has been tested by imaging a MCF7 cell surface.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d7ed62ad16d1114f7e48becf46b3de54 https://doi.org/10.1063/1.1409586 Zobrazit plný text záznamu