RNA interference-mediated knockdown of odorant-binding protein 2 and MP58 gene causes mortality in Myzus persicae
| Autor: | Umer Rashid, Muhammad Faheem Malik, Muhammad Azmat Ullah Khan, Sadia Alam, Muhammad Ramzan Khan, Nadia Zeeshan, Naeem Mehmood Ashraf, Amber Afroz, Iqra Mahmood |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Gene knockdown food and beverages Biology biology.organism_classification 010603 evolutionary biology 01 natural sciences Molecular biology Homology (biology) Reverse transcriptase 010602 entomology RNA silencing Real-time polymerase chain reaction RNA interference Insect Science Myzus persicae Gene Ecology Evolution Behavior and Systematics |
| Zdroj: | International Journal of Tropical Insect Science. 42:315-326 |
| ISSN: | 1742-7592 |
| Popis: | The objective of the study was to find out odorant-binding protein 2 and salivary protein MP58 as potential RNAi targets in Myzus persicae. These potential targets were confirmed by reverse transcriptase polymerase chain reaction. Genes were sequenced and GenBank IDs allotted were MN183129 for odorant-binding protein 2 and MN183130 for MP58. ERNAi tool designed more than 250 siRNA targets from which seven were mentioned with structural map positions for both genes without any off-target. Maximum mortality was observed with odorant-binding protein 2 (70%); followed by MP58 (57%) in comparison to $$\underline{\mathbf{+ve}}$$ and $$\underline{\mathbf{-ve}}$$ control 8d after dsRNA feeding (15 ng/μL). Quantitative polymerase chain reaction expressed the reduced expression of odorant-binding protein 2 (33%) followed by MP58 (40%) gene 8Ds after dsRNA feeding in comparison to control (98%). Phylogenetic analysis exhibited homology of M. persicae odorant-binding protein 2 to grain, oat, pea and corn aphid and MP58 with pea and corn aphid; broaden its application for main staple crops and more specifically for M. persicae. |
| Databáze: | OpenAIRE |
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