Lysine decarboxylase transgenic tobacco root cultures biosynthesize novel hydroxycinnamoylcadaverines

Autor:	Jochen Berlin, L. F. Fecker, C. Mollenschott, S. Herminghaus
Rok vydání:	1998
Předmět:	Cadaverine biology Lysine decarboxylase Nicotiana tabacum Anabasine Plant Science General Medicine Horticulture biology.organism_classification Biochemistry chemistry.chemical_compound Tissue culture chemistry Cauliflower mosaic virus Secondary metabolism Polyamine Molecular Biology
Zdroj:	Phytochemistry. 48:79-84
ISSN:	0031-9422
DOI:	10.1016/s0031-9422(97)01098-4
Popis:	Expression of a bacterial lysine decarboxylase (under the control of the 35S-promoter of the cauliflower mosaic virus and fused to the coding sequence of the small subnit rbcS transit peptide) in tobacco root cultures can affect at least two different secondary pathways. The overproduction of cadaverine by a line with high lysine decarboxylase activity led not only to greatly enhanced accumulation of the minor alkaloid anabasine but also to the formation of hydroxycinnamoylcadaverines, new metabolites which have not yet been described as natural constituents of tobacco. The transgenic root culture was changed into a suspension culture and also plants were regenerated from the root culture. The differences in the metabolic fate of cadaverine in the different morphological states are described. The advantages of systems like this for studying metabolic engineering of plant secondary pathways is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d7d97fbcd5f08aea4c93a76a353a05de https://doi.org/10.1016/s0031-9422(97)01098-4 Zobrazit plný text záznamu Full Text from ScienceDirect