Oryza sativa RNA-Dependent RNA Polymerase 6 Contributes to Double-Strand Break Formation in Meiosis
| Autor: | Huili Wen, Fei Mao, Jiawen Cheng, Zhukuan Cheng, Guijie Du, Wenqing Shi, Yafei Li, Yufeng Wu, Ding Tang, Baoxiang Qin, Yi Shen, Changzhen Liu |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Regulation of gene expression Small RNA Wild type RNA-dependent RNA polymerase RNA Cell Biology Plant Science Biology 01 natural sciences Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry RNA polymerase DNA methylation Gene 010606 plant biology & botany |
| Zdroj: | The Plant Cell. 32:3273-3289 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.20.00213 |
| Popis: | RNA-dependent RNA polymerase 6 (RDR6) is a core component of the small RNA biogenesis pathway, but its function in meiosis is unclear. Here, we report a new allele of OsRDR6 (Osrdr6-meiosis [Osrdr6-mei]), which causes meiosis-specific phenotypes in rice (Oryza sativa). In Osrdr6-mei, meiotic double-strand break (DSB) formation is partially blocked. We created a biallelic mutant with more severe phenotypes, Osrdr6-bi, by crossing Osrdr6-mei with a knockout mutant, Osrdr6-edit. In Osrdr6-bi meiocytes, 24 univalents were observed, and no histone H2AX phosphorylation foci were detected. Compared with the wild type, the number of 21-nucleotide small RNAs in Osrdr6-mei was dramatically lower, while the number of 24-nucleotide small RNAs was significantly higher. Thousands of differentially methylated regions (DMRs) were discovered in Osrdr6-mei, implying that OsRDR6 plays an important role in DNA methylation. There were 457 genes downregulated in Osrdr6-mei, including three genes, CENTRAL REGION COMPONENT1, P31comet, and O. sativa SOLO DANCERS, related to DSB formation. Interestingly, the downregulated genes were associated with a high level of 24-nucleotide small RNAs but less strongly associated with DMRs. Therefore, we speculate that the alteration in expression of small RNAs in Osrdr6 mutants leads to the defects in DSB formation during meiosis, which might not be directly dependent on RNA-directed DNA methylation. |
| Databáze: | OpenAIRE |
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