Immunocytochemical evidence for the involvement of golgi apparatus in the deposition of seed lectin ofBauhinia purpurea (Leguminosae)

Autor:	L. M. Shannon, E. M. Herman
Rok vydání:	1984
Předmět:	Bauhinia Vesicle Immunocytochemistry Lectin Cell Biology Plant Science General Medicine Biology Golgi apparatus biology.organism_classification symbols.namesake Biochemistry Colloidal gold Protein body symbols biology.protein Secretion
Zdroj:	Protoplasma. 121:163-170
ISSN:	1615-6102 0033-183X
DOI:	10.1007/bf01282309
Popis:	The seed lectin of the tree legume,Bauhinia purpurea alba, was localized by electron microscopic immunocytochemistry. The pattern of lectin deposition and site of intracellular localization was examined in mid- to late-maturation seeds. The seed tissue was embedded in Lowicryl K4M, the use of which with seed tissues is discussed. Immunocytochemical labeling was accomplished with colloidal gold coupled to a second antibody. The immunocytochemical reaction was specific and sensitive. Protein bodies, Golgi apparatus and Golgi secretion vesicles were densely labeled. Golgi apparatus was oriented such that Golgi secretion vesicles were in close proximity to the protein bodies. The entire Golgi apparatus was labeled with no concentration gradient across the Golgi stack. These observations indicate that the final site of lectin deposition is the protein body, and that the Golgi apparatus plays an essential role in the deposition process.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d7be255f2ec2ca7089956296dee95a57 https://doi.org/10.1007/bf01282309 Zobrazit plný text záznamu Full text from SpringerLink