Bacillus thuringiensis var.israelensis crystal hemolysis as a possible basis for an assay of larval toxicity

Autor:	N. Sandler, A. Keynan, Joel Margalit, R. Zomper
Rok vydání:	1985
Předmět:	Bacillaceae Toxin Biological activity Hemolysin General Medicine Biology biology.organism_classification medicine.disease_cause medicine.disease Applied Microbiology and Biotechnology Bacillales Hemolysis Microbiology Bacillus thuringiensis medicine Bacteria Biotechnology
Zdroj:	Applied Microbiology and Biotechnology. 23:47-53
ISSN:	1432-0614 0175-7598
DOI:	10.1007/bf02660117
Popis:	Optimal conditions for the extraction of a hemolysin from crystals ofBacillus thurin-giensis var.israelensis (B.t.i.) have been described. Evidence is given that the mechanism of hemoly-sin release from crystals involves proteolytic en-zymes. The hemolysin extracted from B.t.i. crys-tals has been shown to be antigenically different from the hemolysin excreted by vegetative cells of the same strain. As there is good correlation be-tween the hemolytic and larvicidal properties of various B.t.i. preparations it is suggested that the hemolytic assay should be tried as a rapid assay for preliminary determinations of the activity of B.t.i. preparations.
Databáze:	OpenAIRE
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