Exploring the Role of the NO-Detoxifying Enzyme HmpA in the Evolution of Domesticated Alfalfa Rhizobia
| Autor: | Gabriela Soto, Luisa Galindo-Sotomonte, Cecilia Pascuan, Romina Frare, Wayne McCormick, Nicolás Ayub |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Genetics Genome instability Ecology biology Point mutation fungi 030106 microbiology food and beverages Soil Science biochemical phenomena metabolism and nutrition biology.organism_classification Genetic analysis Rhizobia 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Symbiosis Microbial inoculant Gene Ecology Evolution Behavior and Systematics DNA |
| Zdroj: | Microbial Ecology. 83:501-505 |
| ISSN: | 1432-184X 0095-3628 |
| DOI: | 10.1007/s00248-021-01761-4 |
| Popis: | We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present note, we describe the molecular mechanism involved in the evolution of alfalfa rhizobia. Genomic analysis showed that most of the gene losses in inoculants are due to large genomic deletions rather than to small deletions or point mutations, a fact consistent with recurrent DNA double-strand breaks (DSBs) at numerous locations throughout the microbial genome. Genetic analysis showed that the loss of the NO-detoxifying enzyme HmpA in inoculants results in growth inhibition and high DSB levels under nitrosative stress, and large genomic deletions in planta but not in the soil. Therefore, besides its known function in the effective establishment of the symbiosis, HmpA can play a critical role in the preservation of the genomic integrity of alfalfa rhizobia under host-derived nitrosative stress. |
| Databáze: | OpenAIRE |
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