Loss of heterozygosity at individual loci in Arabidopsis thaliana regenerants cultured with para-fluorophenylalanine
| Autor: | M. F. Parii, O. V. Zimina, O. G. Alkhimova |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics biology Callus formation fungi Cell Biology biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Genome Loss of heterozygosity Chromosome segregation 03 medical and health sciences 030104 developmental biology Genetic marker Arabidopsis Arabidopsis thaliana Hybrid |
| Zdroj: | Cytology and Genetics. 50:278-284 |
| ISSN: | 1934-9440 0095-4527 |
| Popis: | Precise chromosome segregation is vital for speciation and hybrid formation. The aim of this work was to study the chromosomes behavior and inheritance of maternal and paternal genomes in Arabidopsis regenerants obtained from in vitro cultured cells on the medium with para-fluorophenyalanine (PFPA). The Arabidopsis thaliana model hybrid between Columbia and Landsberg erecta ecotypes was developed, which chromosomes were easy to distinguish using the 12 SSLP selected markers. Also, the influence of PFPA on callus formation and regeneration of plants was analyzed. 20 regenerated plants cultured with PFPA were derived, three of which were shown to loss the heterozygosity in six loci by DNA markers analysis. Different models are certainly required to understand how and when the mechanisms leading to proper chromosome segregation are established in species and hybrids. |
| Databáze: | OpenAIRE |
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