Selective Butyrate Esterase Probe for the Rapid Colorimetric and Fluorogenic Identification of Moraxella catarrhalis

Autor:	Yeon Ho Kim, Youngmi Kim, Ki Sun Yoon, Uisung Lee
Rok vydání:	2020
Předmět:	biology Butyrate biology.organism_classification Coumarin Esterase Fluorescence Analytical Chemistry Moraxella catarrhalis chemistry.chemical_compound Butyrate esterase chemistry Biochemistry BODIPY Bacteria
Zdroj:	Analytical Chemistry. 92:16051-16057
ISSN:	1520-6882 0003-2700
DOI:	10.1021/acs.analchem.0c03671
Popis:	Clinical identification of the pathogenic bacterium Moraxella catarrhalis in cultures relies on the detection of bacterial butyrate esterase (C4-esterase) using a coumarin-based fluorogenic substrate, 4-methylumbelliferyl butyrate. However, this classical probe may give false-positive responses because of its poor stability and lack of specificity. Here, we report a new colorimetric and fluorogenic probe design employing a meso-ester-substituted boron dipyrromethene (BODIPY) dye for the specific detection of C4-esterase activity expressed by M. catarrhalis. This new probe has resistance to nonspecific hydrolysis that is far superior to the classical probe and also selectively responds to esterase with rapid colorimetric and fluorescence signal changes and large "turn-on" ratios. The probe was successfully applied to the specific detection of M. catarrhalis with high sensitivity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d799a0f9e3fc6b2cb8fa8315743cb625 https://doi.org/10.1021/acs.analchem.0c03671 Zobrazit plný text záznamu