Connectin, an Elastic Protein of Muscle
| Autor: | Koscak Maruyama, Kazuho Ozaki |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Plasmodium (life cycle)
biology Physarum polycephalum General Medicine biology.organism_classification Biochemistry law.invention Cell membrane medicine.anatomical_structure law Cytoplasm parasitic diseases medicine biology.protein Biophysics Titin Electron microscope Cytoskeleton Molecular Biology Actin |
| Zdroj: | The Journal of Biochemistry. 88:883-888 |
| ISSN: | 1756-2651 0021-924X |
| DOI: | 10.1093/oxfordjournals.jbchem.a133043 |
| Popis: | A completely sodium dodecyl sulfate-insoluble protein was obtained from plasmodia of Physarum polycephalum. The amino acid composition of this protein was very similar to that of connectin, an elastic protein of muscle. When the protein suspension was treated with 6 M guanidine-HCl in the presence of 0.1 M 2-mercaptoethanol, entanglements of very thin filaments, less than 5 nm in width, were observed under an electron microscope. Since the network structure is almost the same as that of isolated muscle connectin, this plasmodium protein can be regarded as forming an elastic net structure. Using an antiserum against the insoluble protein, it appeared to be located at the peripheral region of the plasmodium. This elastic net structure may act as a cytoskeleton at the cytoplasmic surface of the plasmodium cell membrane. |
| Databáze: | OpenAIRE |
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