Application of Isozyme Data in Fingerprinting Napier Grass (Pennisetum purpureum Schum.) for Germplasm Management

Autor:	Anitha P. Bhandari, C. R. Ramesh, D. H. Sukanya
Rok vydání:	2006
Předmět:	Germplasm Perennial plant biology Tropics Plant Science Subtropics biology.organism_classification Malate dehydrogenase Isozyme Horticulture Polymorphism (computer science) Botany Genetics Pennisetum purpureum Agronomy and Crop Science Ecology Evolution Behavior and Systematics
Zdroj:	Genetic Resources and Crop Evolution. 53:253-264
ISSN:	1573-5109 0925-9864
DOI:	10.1007/s10722-004-6120-2
Popis:	Sixty-four accessions of Napier grass (Pennisetum purpureum Schum.), a forage crop of tropics and subtropics, were assessed for polymorphism in isozymes and total proteins (TP). The national germplasm collection exhibited polymorphism in all the three isozymes viz., malate dehydrogenase, glutamate oxaloacetate transaminase and peroxidase (POX) besides TP profile. Though POX and TP showed comparatively higher polymorphism, the study indicated the efficiency of three-enzyme system in identifying more than 90% of the accessions. The combination of TP and three-enzyme systems provided unique isozyme/TP banding patterns for all the 64 accessions leading to development of accession specific profile/fingerprints. In Napier, which is perennial with long vegetative phase, it is difficult to discriminate germplasm accessions based only on morphology. Thus biochemical markers could be efficiently utilized to complement morphological evaluations, in maintaining identity and purity of germplasm for proper conservation and management, for better use in breeding and for proprietary reasons.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d7879580c1ad6d98fa831bacfec58094 https://doi.org/10.1007/s10722-004-6120-2 Zobrazit plný text záznamu Full text from SpringerLink