Isolation of Weissella cibaria from Pacific White Shrimp (Litopenaeus vannamei) Gastrointestinal Tract and Evaluation of Its Pathogenic Bacterial Inhibition
| Autor: | Nguyen Thi Dong Phuong, Truong Thi Phuong Lan, Le My Tieu Ngoc, Nguyen Hoang Loc, Nguyen Duc Huy, Hoang Tan Quang, Tran Thuy Lan, Tran Quoc Dung, Vu Duc Hoang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Multidisciplinary
biology Vibrio parahaemolyticus Chlortetracycline Hydrochloride Pathogenic bacteria 02 engineering and technology medicine.disease_cause biology.organism_classification Vibrio Microbiology 030507 speech-language pathology & audiology 03 medical and health sciences Staphylococcus aureus 0202 electrical engineering electronic engineering information engineering medicine 020201 artificial intelligence & image processing Weissella cibaria 0305 other medical science Escherichia coli Bacteria |
| Zdroj: | Indian Journal of Science and Technology. 13:1200-1212 |
| ISSN: | 0974-5645 0974-6846 |
| DOI: | 10.17485/ijst/2020/v13i10/149934 |
| Popis: | Objective: The present study reported the isolation of lactic acid bacteria from the shrimp gastrointestinal tract and evaluation of its antagonistic activity against pathogenic bacteria. Methods/ statistical analysis: Lactic bacteria from the gastrointestinal tract of alive healthy shrimp were screened on sterilized MRS medium. Vibrio sp. inhibition by isolates was conducted by the agar-well diffusion assay. The isolates were identified by 16S rRNA sequencing. Antibacterial activity was investigated against 25 Vibrio sp. isolates, Staphylococcus aureus ATCC 25023, and Escherichia coli ATCC 85922. Hemolytic activity, salt, and antibiotic resistance were also evaluated. Findings: Total of 22 isolates exhibited Vibrio parahaemolyticus inhibition with antagonistic activities ranged from 220 AU/ml to 460 AU/ml. Sequence analysis of 16S rRNA indicated the 5 isolates belong to Weissella cibaria species, namely HN02, HN03, HN04, and HN06 AND HN07. Among these, W. cibaria HN05 had highest antagonistic activity and exhibited the broad inhibition spectrum against Vibrio sp. as well as E. coli ATCC 85922 but not S. aureus ATCC 25023. β-hemolytic and salt resistance assays showed that W. cibaria HN05 had no β-hemolytic activity and grew well in medium containing 5% of salt. Antibiotic resistance analysis indicated W. cibaria HN05 was suppressed by ampicillin, chloramphenicol, oxytetracycline hydrochloride, and chlortetracycline hydrochloride. Application/improvements: This is the first study on isolation and evaluation pathogenic bacteria including Vibrio sp. inhibition by W. cibaria, which is isolated from shrimp gastrointestinal tract. Keywords: Antagonistic Activity, Gastrointestinal Tract, Shirmp, Weissella cibaria, Vibrio sp. |
| Databáze: | OpenAIRE |
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