Experimental models for creation of transgenic plants resistant to stressors

Autor:	N. Mirakhorli, I. V. Goldenkova-Pavlova, E. Isaenko, A. R. Maali, N. A. Kartel, N. O. Yur’eva, I. A. Abdeeva
Rok vydání:	2007
Předmět:	Genetics Hybrid gene biology Transgene food and beverages Cell Biology Genetically modified crops Computational biology biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Human genetics Molecular analysis Bacillus thuringiensis Gene sequence Gene
Zdroj:	Cytology and Genetics. 41:167-171
ISSN:	1934-9440 0095-4527
DOI:	10.3103/s009545270703005x
Popis:	Experimental models of primary potato transgenic plants that express the cry3aM-licBM2 hybrid gene were created. The molecular analysis and biotests of the experimental models allow a new system of cry genes expression in plants to be proposed. This system is based on the expression of hybrid genes containing the reporter lichenase gene sequence and the use of a light-induced promoter ensuring preferential expression of the regulated genes only in green plant tissues (leaves), the target tissues for pests, as a regulatory element. In is shown that the presence of lichenase in hybrid proteins facilitates selection and analysis of the level of expression of hybrid proteins in transgenic plants. Judging by the properties of the reporter protein lichenase in hybrid proteins, it seems possible to use this reporter system for transgene monitoring in agrocenosis, because this system is fairly simple and precise and does not need considerable material and time expenses.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d77c21ca30ce02aa38c02e10d4527edd https://doi.org/10.3103/s009545270703005x Zobrazit plný text záznamu Full text from SpringerLink