Separation and characteristics of different mucopolysaccharides from bovine trachea cartilage
| Autor: | M. Sadowska, J. Łagocka, J. Synowiecki |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
chemistry.chemical_classification
congenital hereditary and neonatal diseases and abnormalities Chromatography nutritional and metabolic diseases General Medicine Polysaccharide Bovine Cartilage Hexosamines Analytical Chemistry Glycosaminoglycan chemistry.chemical_compound Papain Hydrolysis chemistry Hyaluronic acid Trichloroacetic acid skin and connective tissue diseases Food Science |
| Zdroj: | Food Chemistry. 60:533-536 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/s0308-8146(97)00026-5 |
| Popis: | Bovine trachea cartilage may be used as a source of mucopolysaccharides (MPS) for medical and cosmetic purposes. The content of MPS in bovine cartilage ranged from 10 to 11%, on a dry weight basis. Grade MPS isolation involved hydrolysis of the tissue with papain at 60 °C for 24 h at a tissue to enzyme solution ratio of 1:3 ( w v ), separation of enzyme and some peptides with trichloroacetic acid and subsequent precipitation of MPS with ethanol. The MPS contained 54% of polysaccharides and about 27% of residual peptides. The crude MPS were purified and separated into four fractions by precipitation with ethanol at concentrations in reaction media of 20, 30, 40 and 66% ( v v ). These fractions contributed 88, 16.5, 1.5 and 8%, respectively, to the hexosamines present in crude MPS. Total MPS recovery in all fractions was 71% of crude MPS weight. The main components of fractions I, II and IV were chondroitin sulphates, hyaluronic acid, and keratosulphates, respectively. However, fraction III contained 1.6% of total MPS of an unidentified polysaccharide. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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