Fluorescent high-performance liquid chromatography of folic acid and its derivatives using permanganate as a fluorogenic reagent

Autor:	Shigero Ikeda, Mikako Kato, Toshihiro Tsuneyoshi, Tokinori Suzuki, Norio Ichinose
Rok vydání:	1993
Předmět:	chemistry.chemical_compound Chromatography Folic acid Chemistry Present method Reagent Permanganate Pteroylglutamic acid Biochemistry High-performance liquid chromatography Fluorescence Biological fluid Analytical Chemistry
Zdroj:	Fresenius' Journal of Analytical Chemistry. 346:841-846
ISSN:	1618-2650 0937-0633
DOI:	10.1007/bf00321302
Popis:	A fluorescent high-performance liquid chromatography (HPLC) analysis of folic acid (pteroylglutamic acid) and its derivatives using permanganate as a fluorogenic reagent has been developed. In the present work, the various analytical conditions in which the fluorogenic reaction with permanganate could be applied to the HPLC of folic acid in human serum, have been studied. The present method may be employed for the rapid clinical analysis of small amounts of folic acid (≥150 ppb), without interference from coexisting natural fluorophors; the precision of the present method for folic acid was 6.6% (n=6) (coefficient of variation).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d765884a6aa22c80326d6d430d87f3bd https://doi.org/10.1007/bf00321302 Zobrazit plný text záznamu Full text from SpringerLink