Production of bioplastic (poly-3-hydroxybutyrate) using waste paper as a feedstock: Optimization of enzymatic hydrolysis and fermentation employing Burkholderia sacchari
| Autor: | Saif N. Al-Bahry, Huda Al-Battashi, Jay Prakash Verma, Anu Sadasivan Nair, Nallusamy Sivakumar, Neelamegam Annamalai, Shatha Al-Kindi |
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| Rok vydání: | 2019 |
| Předmět: |
Municipal solid waste
biology Renewable Energy Sustainability and the Environment Chemistry 020209 energy Strategy and Management 05 social sciences Biomass 02 engineering and technology Raw material Pulp and paper industry biology.organism_classification Bioplastic Industrial and Manufacturing Engineering Hydrolysate Polyhydroxybutyrate Enzymatic hydrolysis Burkholderia sacchari 050501 criminology 0202 electrical engineering electronic engineering information engineering 0505 law General Environmental Science |
| Zdroj: | Journal of Cleaner Production. 214:236-247 |
| ISSN: | 0959-6526 |
| DOI: | 10.1016/j.jclepro.2018.12.239 |
| Popis: | The global demand for bio-plastic particularly polyhydroxyalkanoate (PHA) have been increased in the last few decades as a substitute of petrochemical-based plastic. Utilization of waste paper, the primary constituent of municipal solid waste (MSW), as a carbon source for polyhydroxybutyrate (PHB) production is not only an alternative, environmental friendly route of waste management but also helps to valorize the waste. In this study, hydrogen peroxide pretreated waste paper saccharification has been optimized using central composite design (CCD). The maximum hydrolysis (88.18%) occurred at paper loading 5.0 g/L, agitation 242 rpm, working volume 20%, cellulase 49.82 U/g, β-glucosidase 20.9 U/g and hemicellulase 29.5 U/g. PHB synthesis and biomass accumulation by xylose-utilizing Burkholderia sacchari using waste paper hydrolysate were studied using different nitrogen sources and carbon to nitrogen (C/N) ratios. Maximum PHB and dry cell weight (DCW) occurred with ammonium sulfate and a C/N ratio of 20. The highest biomass (3.63 g/L), the maximum PHB accumulation (44.2%) and the maximum reducing sugar utilization (92.1%) were observed after 48 h of cultivation using diluted hydrolysate. The physicochemical properties of the extracted PHB were compatible with the standard PHB. Hence, the waste paper could be exploited as a renewable feedstock for the sustainable production of PHB. |
| Databáze: | OpenAIRE |
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