Isolation and Preliminary Characterization of Chalcone Ro 09-0410-Resistant Human Rhinovirus Type 2
| Autor: | Salem R. Yasin, D. A. J. Tyrrell, W. Al-Nakib |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
0301 basic medicine
Chalcone biology Stereochemistry 030106 microbiology Mutant General Medicine Oxime 01 natural sciences In vitro 0104 chemical sciences 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound chemistry Mechanism of action Polyclonal antibodies medicine biology.protein medicine.symptom Mode of action Cytopathic effect |
| Zdroj: | Antiviral Chemistry and Chemotherapy. 1:149-154 |
| ISSN: | 2040-2066 |
| DOI: | 10.1177/095632029000100210 |
| Popis: | Summary We have isolated a human rhinovirus type-2 (HRV-2) mutant that is resistant to the antiviral agent chalcone Ro 09-0410 (4'-ethoxy-2'-hydroxy-4, 6'-dimethoxy chalcone). This Ro 09-0410-resistant HRV-2 mutant (SR2-0410) exhibited altered biological properties when compared with the parental wild-type (wt) HRV-2. It was unstable when exposed to acid and heat in the presence of the drug, was incapable of produc ing plaques and produced an early cytopathic effect (OPE) at high temperatures (35°0 and 37°0). Further more, compared with the parental wild-type, it showed a reduced ability to be neutralized by an anti-HRV-2 polyclonal serum and monoclonal antibodies. This SR2-0410 mutant demonstrated cross-resistance to other synthetic anti-rhinovirus compounds, which are also thought to bind to the viral capsid protein (VPI), such as 4' ,6-dichloroflavan, 3-methoxy-6-[4-(3-methyl phenyQ- I-piperazinyl] pyridazine (R 61837) and WIN 51711 (5-[7-[4-(4,5-dihydro-2-oxazolyl) phenoxyl] heptyl]-3-methyl-isoxazole). Furthermore, it was also resistant to various antiviral combinations synergistic against HRV-2. However, it was still sensitive to enviroxime [2-amino-I-(isopropyl sulphonyl)-6-benzi midazole phenyl ketone oxime], which has a different mode of action and is thought to interfere with viral RNA synthesis. |
| Databáze: | OpenAIRE |
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