| Autor: | Lisandro Giraldez, Elena Girardi, G. Rodríguez de Lores Arnaiz, Gabriela Vanore |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Central nervous system Hippocampus General Medicine Biology Hippocampal formation Ligand (biochemistry) Biochemistry Adenosine Cellular and Molecular Neuroscience chemistry.chemical_compound Adenosine A1 receptor Endocrinology medicine.anatomical_structure chemistry Internal medicine CCPA medicine Convulsant medicine.drug |
| Zdroj: | Neurochemical Research. 26:225-230 |
| ISSN: | 0364-3190 |
| DOI: | 10.1023/a:1010912516299 |
| Popis: | Specific ligand binding to rat hippocampal adenosine A1 receptor after administration of the convulsant drug 3-mercaptopropionic acid (MP) was studied by means of a quantitative autoradiographic method. 2-Chloro-N6-[cyclopentyl-2,3,4,5-3H adenosine] ([3H]CCPA), a potent and selective A1 receptor ligand, was selected for binding studies. MP administration (150 mg/kg, i.p.), at seizure, caused significant increases in the following CA1 layers: pyramidal (45%), radiatum (18%) and lacunosum molecular (35%); in CA2 area, a significant decrease in stratum oriens (36%) and an increase in stratum radiatum (14%) and lacunosum molecular (33%) layers was observed. In CA3 area a rise in pyramidal (40%) and radiatum layers (26%), as well as in hillus (97%) was found. At postseizure, changes were restricted to CA1, CA2 and CA3 pyramidal layers and to CA1 lacunosum molecular layer, with increases ranging from 22 to 50%. These results show that [3H]CCPA binding is modified diversely in intrahippocampal layers and areas, thus indicating their dissimilar role in seizure activity. |
| Databáze: | OpenAIRE |
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