| Autor: |
Kiara N. Berríos, Peng Hu, Connolly Dr, Timothy C. Wang, Emily B. Fabyanic, Kohil Rm, Jennifer Flournoy, Zhaolan Zhou, Qi Qiu, Hao Wu |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.03.23.434325 |
| Popis: |
Oxidative modification of 5-methylcytosine (5mC) generates 5-hydroxymethylcytosine (5hmC), a DNA modification that exhibits unique epigenetic regulatory functions and impacts diverse biological processes. However, standard single-nucleus/cell bisulfite sequencing methods cannot resolve the base ambiguity between 5mC and 5hmC to accurately measure cell-type specific epigenomic patterns and gene regulatory functions of 5hmC or true 5mC. Here, we develop single-nucleus 5hmC sequencing (snhmC-seq) for quantitative and unbiased 5hmC profiling in single cells by harnessing differential deaminase activity of APOBEC3A towards 5mC and chemically protected 5hmC. We used snhmC-seq to profile single nuclei from cryopreserved mouse brain samples to reveal epigenetic heterogeneity of 5hmC at single-cell resolution and uncovered a non-canonical gene regulatory role of genic 5hmC in non-CG context. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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