Improving the Selectivity and Confidence in the HPLC Analysis of Microcystins in Lake Sediments

Autor:	Christoph Schmidtkunz, Thomas Welsch, Hans Bernd Stich
Rok vydání:	2009
Předmět:	chemistry.chemical_classification Chromatography Chemistry Aquatic ecosystem Clinical Biochemistry Pharmaceutical Science Sediment Microcystin Biochemistry Analytical Chemistry Gel permeation chromatography Matrix (chemical analysis) Congener Environmental chemistry Water pollution Eutrophication
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 32:801-821
ISSN:	1520-572X 1082-6076
Popis:	HPLC is a well established method for the congener selective analysis of microcystins (potent cyanobacterial hepatotoxins) in water samples or cyanobacterial cells. Its application to microcystin determination in sediments from aquatic ecosystems is still an emerging technique, often hampered by the inherent complexity of the matrix “sediment” that may lead to selectivity (and hence confidence) issues due to coeluting substances. Using size exclusion chromatography cleanup, we could demonstrate that the confidence of microcystin analysis in sediments by HPLC with UV detection can be enhanced, yet the improvements are not always sufficient to yield unambiguous results. This drawback was avoided by employing mass spectrometric detection hyphenated to HPLC. The developed analysis protocol was applied to organic rich, silty sediments from Lake Federsee, a eutrophic lake in Southwestern Germany. Results from these experiments are presented, along with a discussion of artefacts arising from the esterif...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d744436633068e4c42d1e15472a283af https://doi.org/10.1080/10826070902767999 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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