In vivo formation of sesquiterpene hydrocarbons in the endoplasmic reticulum of pine

Autor: Ginette Pauly, M. Gleizes, Colette Bernard-Dagan, Jean-Pierre Carde
Rok vydání: 1980
Předmět:
Zdroj: Plant Science Letters. 20:79-90
ISSN: 0304-4211
DOI: 10.1016/0304-4211(80)90026-7
Popis: Memebrane fractions were isolated from an homogenate of primary leaves of maritime pine (Pinus pinaster Ait.) seedlings, labelled in vivo by [1-14C]acetate. The various membranes from the 38 000 × g pellet were isolated by discontinuous density gradient, characterized with enzymic markers and identified by electron microscopy. It is shown that two fractions containing labelled sesquiterpene hydrocarbons were enriched in endoplasmic reticulum. A fraction devoid of radioactivity located between these fractions is very probably enriched in tonoplast.
Databáze: OpenAIRE