Promoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-ε-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton’s Jelly:In VitroandIn VivoAnalysis
| Autor: | Ana Colette Maurício, A. L. Luís, Paulo Jorge Da Silva Bartolo, Tiago Pereira, José D. Santos, Ana Caseiro, A S P Varejão, Andrea Gartner, Federica Fregnan, Irina Amorim, André Almeida, Paulo Armada-da-Silva, Sandra Amado, Stefano Geuna |
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| Rok vydání: | 2014 |
| Předmět: |
0303 health sciences
Wallerian degeneration General Immunology and Microbiology Regeneration (biology) Cellular differentiation Mesenchymal stem cell General Medicine Anatomy Biology medicine.disease General Biochemistry Genetics and Molecular Biology Cell biology Neurotmesis 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Wharton's jelly medicine Neuroglia Sciatic nerve 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | BioMed Research International. 2014:1-17 |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | In peripheral nerves MSCs can modulate Wallerian degeneration and the overall regenerative response by acting through paracrine mechanisms directly on regenerating axons or upon the nerve-supporting Schwann cells. In the present study, the effect of human MSCs from Wharton’s jelly (HMSCs), differentiated into neuroglial-like cells associated to poly (DL-lactide-ε-caprolactone) membrane, on nerve regeneration, was evaluated in the neurotmesis injury rat sciatic nerve model. Resultsin vitroshowed successful differentiation of HMSCs into neuroglial-like cells, characterized by expression of specific neuroglial markers confirmed by immunocytochemistry and by RT-PCR and qPCR targeting specific genes expressed.In vivotesting evaluated during the healing period of 20 weeks, showed no evident positive effect of HMSCs or neuroglial-like cell enrichment at the sciatic nerve repair site on most of the functional and nerve morphometric predictors of nerve regeneration although the nociception function was almost normal. EPT on the other hand, recovered significantly better after HMSCs enriched membrane employment, to values of residual functional impairment compared to other treated groups. When the neurotmesis injury can be surgically reconstructed with an end-to-end suture or by grafting, the addition of a PLC membrane associated with HMSCs seems to bring significant advantage, especially concerning the motor function recovery. |
| Databáze: | OpenAIRE |
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