The Effect of Helium on Cryopreservation of HeLa and L929 Cells
| Autor: | E. E. Fesenko, E. L. Gagarinskiy, N. E. Shvirst, S. V. Ugraitskaya, L. V. Zalomova, S. A. Kaurova, M. V. Goltyaev, A. L. Kovtun, Natalia V. Shishova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chromatography 030102 biochemistry & molecular biology biology Cryoprotectant Cell Biophysics chemistry.chemical_element respiratory system biology.organism_classification Nitrogen Cryopreservation HeLa 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology medicine.anatomical_structure chemistry Cell culture Glycerol medicine Viability assay |
| Zdroj: | Biophysics. 63:387-392 |
| ISSN: | 1555-6654 0006-3509 |
| Popis: | The effect of helium on cell survival during cryopreservation was studied using the HeLa and L929 cell lines. Cell suspensions were incubated in an atmosphere of helium, nitrogen, or air and frozen in the presence or absence of glycerol as a cryoprotectant. After thawing, the cell viability was evaluated by the Trypan Blue exclusion test and culture development for 18 h. Helium was found to provide better preservation of cell suspensions compared with nitrogen and air. The positive effect of helium was the greatest in the case of freezing without cryoprotectants (the HeLa cell survival increased by a factor of 1.5–2) and somewhat lower in the case of freezing in the presence of low glycerol concentrations (the L929 cell survival increased by a factor of 1.2–1.5 in the presence of 3% glycerol). Use of helium for cell suspensions may improve cryopreservation methods by making it possible to reduce the concentrations of conventional cryoprotectants, which are generally highly toxic and undesirable to use for cryopreservation of biological material for medical applications. |
| Databáze: | OpenAIRE |
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