| Popis: |
1. 1. Of several anticoagulants tested, only N-ethyl maleimide (NEM) prevents lysis of shrimp hemocytes and maintains their normal morphology. 2. 2. Coagulogen, the clotting protein, is a plasma protein with molecular mass of ≈400 kDa. 3. 3. Transglutaminase, the enzyme that cross links coagulagen to form a visible clot, is more abundant in the hemocytes than in plasma. 4. 4. Hemocytes can be separated into two bands by differential centrifugation. The upper band is enriched wity hyaline cells, which initiate clot formation, and contains most of the transglutaminase activity. 5. 5. The mechanisms of hemolymph coagulation are discussed. |
