Genetic Variability of PhytopathogenicFusarium proliferatumAssociated with Crown Rot inAsparagus officinalis
| Autor: | Oliver Martinez, Ines Schadock, Susanne von Bargen, Anne-Mareen Ellen Eisold, Carmen Büttner, Monika Gossmann |
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| Rok vydání: | 2009 |
| Předmět: |
biology
Physiology food and beverages Fusarium proliferatum Plant Science Fungi imperfecti biology.organism_classification chemistry.chemical_compound chemistry Fumonisin Genetic variation Botany Genetics Gibberella Asparagus Genetic variability Restriction fragment length polymorphism Agronomy and Crop Science |
| Zdroj: | Journal of Phytopathology. 157:446-456 |
| ISSN: | 1439-0434 0931-1785 |
| DOI: | 10.1111/j.1439-0434.2008.01525.x |
| Popis: | Fusarium proliferatum (teleomorph: Gibberella inter-media) is a causal agent of crown rot of Asparagus officinalis and is one potential fumonisin-producing species within the genus Fusarium. It colonizes roots and crowns of asparagus plants, but could also be isolated from symptomless asparagus spears. Fusarium proliferatum isolates obtained from perennial asparagus plantings from Austria and Germany were included in a study on detectability and variability of two essential genes of the fumonisin-gene cluster. Genetic fingerprinting of 45 isolates revealed 14 different fingerprint groups, indicating genetic heterogenicity of F. proliferatum. Most isolates differentiated into three main fingerprint clusters, but no association was found between fingerprint group and origin of the isolates. By gene-specific PCR it was shown that, in 25 isolates tested, both initial genes of the fumonisin biosynthetic pathway ― FUM1, encoding a polyketide synthase and FUM8, a gene for a putative aminoacyl transferase ― were detectable. This suggests that these isolates were able to produce fumonisins and could contribute to the detected contamination in originating asparagus spears with this mycotoxin. Thus, early detection of FUM-genes in F. proliferatum-colonized asparagus may be suited to prevent uptake of fumonisin contaminated food with the human diet. Restriction fragment length polymorphism analysis (PCR-RFLP) of the amplified FUM gene fragments revealed little sequence variability, suggesting a conserved structure of these genes within this species. However, sequence analysis confirmed intraspecific nucleotide polymorphisms of these genes. |
| Databáze: | OpenAIRE |
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