Construction and primary analysis of human keratinocyte model expressing anti-sense protein kinase Cζ

Autor:	Huitu Liu, Xiaoyan Zhang, Shengqing Ma
Rok vydání:	1998
Předmět:	Multidisciplinary Cell growth Transfection Biology Molecular biology Cell cycle phase Cell biology Blot Plasmid medicine.anatomical_structure medicine Protein kinase A Keratinocyte Protein kinase C
Zdroj:	Chinese Science Bulletin. 43:1556-1562
ISSN:	1861-9541 1001-6538
DOI:	10.1007/bf02883448
Popis:	Protein kinase C (PKC), which has a wide variety of functions, comprises at least twelve isoenzymes that have distinct roles respectively. Research in recent years indicates that PKCζ plays a pivotal role in the regulation of cell proliferation. In order to investigate its role in the regulation of keratinocyte proliferation, eukaryotic expression plasmid of anti-sense PKCζ is constructed and transfected into human keratinocyte line, Colo16. The human keratinocyte model expressing anti-sense PKCζ is established successfully, the growth properties of the cell model is analyzed by the detection of cell growth curve and cell cycle phase. The mechanism of the relationship between PKCζ and CyclinD1 is also investigated by the western blotting analysis of CyclinDl protein.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d6dc9100205571fe5d4fd600a25763d2 https://doi.org/10.1007/bf02883448 Zobrazit plný text záznamu Full text from SpringerLink