Comparison of quantitative real-time PCR, chitin and ergosterol assays for monitoring colonization of Trametes versicolor in birch wood

Autor:	Ari M. Hietala, Carl Gunnar Fossdal, Gry Alfredsen, Halvor Solheim, Morten Eikenes
Rok vydání:	2005
Předmět:	Ergosterol Chromatography biology Inoculation Betula pubescens biology.organism_classification law.invention Biomaterials chemistry.chemical_compound chemistry Chitin law Hardwood Food science Incubation Polymerase chain reaction Trametes versicolor
Zdroj:	Holzforschung. 59:568-573
ISSN:	1437-434X 0018-3830
Popis:	This paper describes the use of quantitative real-time PCR for monitoring colonization of birch wood (Betula pubescens) by the white-rot fungusTrametes versicolorin an EN113 decay experiment. The wood samples were harvested after 4, 8, 12, 16 and 20 weeks of incubation. The mass loss was in the range of 4–40%. Chitin and ergosterol assays were conducted for comparison. Second-order polynomial fits of the mass loss of decayed wood versus chitin, ergosterol and DNA gave correlations (r2) of 0.87, 0.61 and 0.84, respectively. Compared to the other two assays employed, real-time PCR data correlated best with the relative mass loss of decayed samples 4–8 weeks after inoculation, while the saturation and decline of DNA-based estimates for fungal colonization 16–20 weeks after inoculation indicated that the DNA assay is not suited for quantification purposes in the late stages of decay. The impact of conversion factors, extraction efficiency, inhibitory compounds and background levels in relation to the three detection assays used is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d6c8d0484edc200f30e0f8a25a7f7756 https://doi.org/10.1515/hf.2005.093 Zobrazit plný text záznamu