Nitrate reduction in a new strain ofRhodoferax fermentans

Autor:	Angela Hougardy, Jobst-Heinrich Klemme
Rok vydání:	1995
Předmět:	Rhodobacter biology Strain (chemistry) General Medicine biology.organism_classification Nitrate reductase Biochemistry Microbiology chemistry.chemical_compound Nitrate chemistry Rhodoferax Genetics Rhodospirillales Molecular Biology Rhodospirillaceae Bacteria
Zdroj:	Archives of Microbiology. 164:358-362
ISSN:	1432-072X 0302-8933
DOI:	10.1007/bf02529983
Popis:	Using an enrichment technique avoiding the dominating fast-growing speciesRhodobacter capsulatus andRhodobacter sphaeroides, a new strain (designated B2c; DSM 10 139) ofRhodoferax fermentans, a facultatively phototrophic member of the β subclass of Proteobacteria, was isolated. In contrast to the type strain of this species, strain B2c was capable of reducing nitrate. Strain B2c contained a highly active nitrate reductase [0.3–0.4 μmol min−1 (mg protein)−1] which, in extracts from spheroplasts prepared by ultrasonic treatment, was associated with the membrane fraction. The physiological role of nitrate reductase depended on the growth conditions. The enzyme enabled the strain to grow with nitrate as a nitrogen source and to maintain redox balance in a culture medium with a highly reduced carbon compound.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d6c89d0f5bc6963a654d0e1909458e13 https://doi.org/10.1007/bf02529983 Zobrazit plný text záznamu Full text from SpringerLink