| Autor: |
Sizue Ota Rogero, Monica Beatriz Mathor, Mara T. S. Alcântara, Patricia Santos Lopes, Maria Valéria Robles Velasco, Ademar B. Lugão, Gislaine Zulli |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Radiation Physics and Chemistry. 79:286-288 |
| ISSN: |
0969-806X |
| DOI: |
10.1016/j.radphyschem.2009.08.025 |
| Popis: |
Papain is a proteolytic enzyme that has been widely used as debridement agent for scars and wound healing treatment. However, papain presents low stability, which limits its use to extemporaneous or short shelf-life formulations. The purpose of this study was to entrap papain into a polymeric matrix in order to obtain a drug delivery system that could be used as medical device. Since these systems must be sterile, gamma radiation is an interesting option and presents advantages in relation to conventional agents: no radioactive residues are formed; the product can be sterilized inside the final packaging and has an excellent reliability. The normative reference for the establishment of the sterilizing dose determines 25 kGy as the inactivation dose for viable microorganisms. A silicone dispersion was selected to prepare membranes containing 2% (w/w) papain. Irradiated and non-irradiated membranes were simultaneously assessed in order to verify whether gamma radiation interferes with the drug-releasing profile. Results showed that irradiation does not affect significantly papain release and its activity. Therefore papain shows radioresistance in the irradiation conditions applied. In conclusion, gamma radiation can be easily used as sterilizing agent without affecting the papain release profile and its activity onto the biocompatible device is studied. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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