Impact of Lyophilisation on Integrity of Structural and Functional Characteristics of Human Cord Blood Leukoncentrate
| Autor: | Larisa Sokil, Mykola Bondarovych, L. V. Ostankova, Anatoliy N. Goltsev, Maksim V. Ostankov, Olena Lutsenko, Igor G. Grisha, Ganna Koval, Kateryna Yampolskaya |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Problems of Cryobiology and Cryomedicine. 29:332-343 |
| ISSN: | 2518-7376 2307-6143 |
| Popis: | This paper presents the investigations on the impact of lyophilisation conditions on structural and functional characteristics of human cord blood leukoconcentrate (HCBL) such as: the number and viability, cytomorphological indices, content of hematopoietic stem (CD34+) and T-regulatory cells (CD4+CD25high), hematopoietic colony forming units in culture (CFUc). Four regimens, including two programs with and without 3.5% DMSO were used for hcbl cell lyophilisation. The programs dii¬€ered in temperature value and duration wherein the primary drying proceeded. When using the i¬rst program, the cells were dried for 10 hrs (–28ÂoÐi), and 9 hrs (–18ÂoÐi) for the second one. The designed method for HCBL lyophilisation, suggested the use of the DMSO-free i¬rst program, ensured the cell preservation in a heterogeneous population of cord blood according to all the studied characteristics. Probl Cryobiol Cryomed 2019; 29(4): 332-343 |
| Databáze: | OpenAIRE |
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