Synthesis of drug loaded magnetic nanoparticles and their uptake into immune cells
| Autor: | Ruth Eggers, Rolf Hempelmann, Eva-Marie Prinz, Ute Steinfeld, Hyeck-Hee Lee |
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| Rok vydání: | 2010 |
| Předmět: |
History
Materials science Stereochemistry technology industry and agriculture macromolecular substances Fluorescence Fluorescence spectroscopy Computer Science Applications Education chemistry.chemical_compound Dextran Immune system Dynamic light scattering chemistry Drug delivery Biophysics medicine Magnetic nanoparticles Doxorubicin medicine.drug |
| Zdroj: | Journal of Physics: Conference Series. 200:122009 |
| ISSN: | 1742-6596 |
| DOI: | 10.1088/1742-6596/200/12/122009 |
| Popis: | Ferrite nanoparticles (Mn0,8Zn0,2Fe2O4) are synthesized by the co-precipitation method and characterized by X-ray diffraction, transmission electron microscopy and dynamic light scattering. The particles are functionalized with dextran which is activated via amino or carboxymethyl groups. The chemotherapeutic drug doxorubicin (DOX) is attached to these dextran derivates in different ways. One method is based on the attachment of DOX to amino dextran by its keto group; the other is a bond to the primary amino group of DOX. The characterization of drug loaded dextran derivates is performed by Raman, FT-IR-, UV/VIS-and fluorescence spectroscopy. The biofunctionalized particles are intended for use in adoptive cancer immunotherapy as a new approach, where immune cells (T lymphocytes) will be used as new autonomous highly target specific drug delivery systems. The uptake efficiency of these particles into T lymphocytes is investigated by fluorescence and convocal microscopy. |
| Databáze: | OpenAIRE |
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