Selenium Exerts Protective Effects Against Fluoride-Induced Apoptosis and Oxidative Stress and Altered the Expression of Bcl-2/Caspase Family
| Autor: | Guohua Song, Jiping Gao, Xiaotang Wang, Xiaoru Yan, Xiaoyan Yan, Jianing Wei, Xiaolin Tian, Yu Wang |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Antioxidant Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry chemistry.chemical_element 010501 environmental sciences medicine.disease_cause 01 natural sciences Biochemistry Inorganic Chemistry Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Caspase 0105 earth and related environmental sciences chemistry.chemical_classification 0303 health sciences biology Chemistry Glutathione peroxidase 030302 biochemistry & molecular biology Biochemistry (medical) General Medicine Malondialdehyde Endocrinology Apoptosis biology.protein Selenium Oxidative stress |
| Zdroj: | Biological Trace Element Research. 199:682-692 |
| ISSN: | 1559-0720 0163-4984 |
| DOI: | 10.1007/s12011-020-02185-w |
| Popis: | Fluoride is widely distributed in nature, and at high concentrations, it targets the kidney and especially proximal tubule epithelial cells. Selenium is a typical trace element beneficial to humans, and the role of selenium in the prevention and treatment of fluoride-induced organ damage is an important research topic. The purpose of this study was to investigate the possible protective effects of selenium against fluoride-induced oxidative stress and apoptosis in rat renal tubular epithelial cells. We showed that the activity of antioxidant enzymes (superoxide dismutase and glutathione peroxidase) and total antioxidant capacity were significantly reduced in NaF-treated normal rat kidney cells (NRK-52E), whereas the levels of nitrogen monoxide (NO) and malondialdehyde (MDA) were significantly increased. Moreover, the number of apoptotic cells, mRNA expression of Bax, Bad, caspase-3, caspase-8, and caspase-9, and protein expression of Bax were elevated, while mitochondrial membrane potential and the protein expression of Bcl-2 were reduced. Compared with the NaF group, pretreatment with selenium enhanced the activity of antioxidant enzymes, mitochondrial membrane potential, and protein expression of Bcl-2, while the levels of NO and MDA, number of apoptotic cells, mRNA expression of Bax, Bad, caspase-3, caspase-8, and caspase-9, and protein expression of Bax were decreased. In conclusion, selenium exerted remarkable protective effect against NaF-induced oxidative stress and apoptosis and altered the expression of Bcl-2/caspase family. |
| Databáze: | OpenAIRE |
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