Molecular biological properties of new isolates of plum pox virus strain Winona
Autor: | Alexander Zakubanskiy, S. N. Chirkov, Anna Sheveleva |
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Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Aphid biology Phylogenetic tree Potyviridae ved/biology ved/biology.organism_classification_rank.species Potyvirus food and beverages Nicotiana benthamiana biology.organism_classification 01 natural sciences General Biochemistry Genetics and Molecular Biology Virus 03 medical and health sciences Prunus 030104 developmental biology Botany Brachycaudus cardui General Agricultural and Biological Sciences 010606 plant biology & botany General Environmental Science |
Zdroj: | Moscow University Biological Sciences Bulletin. 71:71-75 |
ISSN: | 1934-791X 0096-3925 |
DOI: | 10.3103/s0096392516020103 |
Popis: | Plum pox virus (PPV, genus Potyvirus, family Potyviridae) is the most important virus pathogen of stone fruit cultures of the genus Prunus from an economical standpoint. Winona strain (PPV-W) is the most variable of nine known virus strains and one of the most widespread in the European part of Russia. Six new PPV-W isolates were first discovered in green plantations of Moscow (Kp2U, Avang, Pulk, Pulk-1), in Taldomsky district of Moscow region (Karm), and in Kovrovsky district of Vladimir region (Vlad-4) on wild trees of plum Prunus domestica. 3’-Terminal segment of genome of new isolates was notable for high variability level. The study on the relationship with other isolates of this strain by means of phylogenetic analysis of gene sequence of the coat protein showed the lack of clusterization of Russian PPV-W isolates according to geographical principle. Inoculation of Nicotiana benthamiana plants by hop plant louse Phorodon humili from plum trees infected with Avang and Pulk isolates and by thistle aphid Brachycaudus cardui from the tree infected with Kp2U isolate led to the systemic viral infection in indicator plants, suggesting the possibility of PPV-W spread by both aphid species in nature. Transmission of PPV-W through seeds was not observed. |
Databáze: | OpenAIRE |
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