Butyrate and propionate production from D-mannitol in the large intestine of pig and rat
| Autor: | Makiko Maekawa, Kazunari Ushida, Seiko Hoshi, Naoko Kashima, Katsumi Ajisaka, Takaji Yajima |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
chemistry.chemical_classification
biology General Engineering Stimulation Butyrate biology.organism_classification Microbiology medicine.anatomical_structure chemistry In vivo Propionate medicine General Earth and Planetary Sciences [Clostridium] indolis Fermentation Large intestine Bacteria General Environmental Science |
| Zdroj: | Microbial Ecology in Health and Disease. 17:169-176 |
| ISSN: | 1651-2235 |
| Popis: | Fermentation of D-mannitol and the bacteria responsible for it were analyzed by 13C-NMR spectroscopy and amplified ribosomal restriction analyses (ARDRA) on intestinal bacteria in pig cecal digesta and in a rat in vivo feeding trial. [1-13C]-Mannitol was fermented by pig cecal digesta mainly to [2-13C]-propionate, [3-13C]-propionate, [2-13C]-butyrate, and [4-13C]-butyrate. Dietary D-mannitol at a 5% level induced significantly higher cecal butyrate concentration in the rat model. The evolution of rat fecal bacteria due to feeding with D-mannitol suggested the involvement of a lactate-utilizing butyrate producer, Clostridium indolis, in the stimulation of butyrate production. Rat intestinal microbiota is often difficult to extrapolate to that of humans, but the stimulation of the lactate-utilizing butyrate producers and propionate producers was suggested by the 13C-NMR spectroscopy of the end products of pig cecal fermentation and ARDRA on rat fecal bacteria. These results suggest the potential of D-mannit... |
| Databáze: | OpenAIRE |
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