Effects of OP-41483·α-CD, a stable prostacyclin analog, on cultured endothelial cell dysfunction caused by 15(S)-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE) in vitro
| Autor: | Ryoji Matsumoto, Masao Naka, Hideki Aishita, Buichi Fujitani, Nagashige Omawari |
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| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Endothelium Eicosatetraenoic acid Cell Prostacyclin General Medicine Biology General Biochemistry Genetics and Molecular Biology In vitro Endothelial stem cell Dose–response relationship chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry Internal medicine medicine General Pharmacology Toxicology and Pharmaceutics Intracellular medicine.drug |
| Zdroj: | Life Sciences. 52:1257-1264 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(05)80015-3 |
| Popis: | We studied the effect of OP-41483.alpha-CD, a stable prostacyclin analog, on, 15-HPETE induced bovine endothelial cell dysfunction, which was assessed by measuring a number of endothelial cells attached to plastic plates. 15-HPETE decreased the number of attached endothelial cells in a concentration- and time-dependent manner. OP-41483.alpha-CD and PGI2 significantly inhibited 15-HPETE induced dysfunction of the cells at a concentration of more than 10(-9)M. Besides, DDA (10(-6) - 10(-4)M) an adenylate cyclase inhibitor, diminished the inhibitory effect of OP-41483.alpha-CD on 15-HPETE induced cell dysfunction in a concentration-dependent manner. Furthermore, OP-41483.alpha-CD increased cAMP levels in the endothelial cells in the range of 10(-10) to 10(-8)M in a dose-dependent manner. These data suggest that OP-41483.alpha-CD could exert an inhibitory action on 15-HPETE induced endothelial cell dysfunction via partly increasing its effect on the intracellular cAMP level. |
| Databáze: | OpenAIRE |
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