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Department of Forensic Chemistry, National Forensic Service Southern District Office, Yangsan-si 626-742, KoreaReceived March 8, 2013, Accepted May 20, 2013Forensic and legal medicine require reliable data to indicate excessive alcohol consumption. Ethanol isoxidatively metabolized to acetate by alcohol dehydrogenase and non-oxidatively metabolized to ethylglucuronide (EtG), ethyl sulfate (EtS), phosphatidylethanol, or fatty acid ethyl esters (FAEE). Oxidativemetabolism is too rapid to provide biomarkers for the detection of ethanol ingestion. However, the non-oxidative metabolite EtG is a useful biomarker because it is stable, non-volatile, water soluble, highly sensitive,and is detected in body fluid, hair, and tissues. EtG analysis methods such as mass spectroscopy, chromato-graphy, or enzyme-linked immunosorbent assay techniques are currently in use. We suggest that nuclearmagnetic resonance (NMR) spectroscopy could be used to monitor ethanol intake. As with currentconventional methods, NMR spectroscopy doesn’t require complicated pretreatments or sample separation.This method has the advantages of short acquisition time, simple sample preparation, reproducibility, andaccuracy. In addition, all proton-containing compounds can be detected. In this study, we performed |
