Cadmium - glutathione solution structures provide new insights into heavy metal detoxification
| Autor: | Emmanuel Godat, Jean Labarre, Christophe Junot, Alain Valleix, Yves Boulard, Hervé Desvaux, Olivier Delalande |
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| Rok vydání: | 2010 |
| Předmět: |
Pollutant
0303 health sciences Cadmium chemistry.chemical_element Cell Biology Glutathione Tripeptide 010402 general chemistry 01 natural sciences Biochemistry 0104 chemical sciences Metal 03 medical and health sciences chemistry.chemical_compound chemistry visual_art Detoxification visual_art.visual_art_medium Chelation Molecular Biology Heavy metal detoxification 030304 developmental biology |
| Zdroj: | FEBS Journal. 277:5086-5096 |
| ISSN: | 1742-464X |
| Popis: | Cadmium is a heavy metal and a pollutant that can be found in large quantities in the environment from industrial waste. Its toxicity for living organisms could arise from its ability to alter thiol-containing cellular components. Glutathione is an abundant tripeptide (γ-Glu-Cys-Gly) that is described as the first line of defence against cadmium in many cell types. NMR experiments for structure and dynamics determination, molecular simulations, competition reactions for metal chelation by different metabolites (γ-Glu-Cys-Gly, α-Glu-Cys-Gly and γ-Glu-Cys) combined with biochemical and genetics experiments have been performed to propose a full description of bio-inorganic reactions occurring in the early steps of cadmium detoxification processes. Our results give unambiguous information about the spontaneous formation, under physiological conditions, of the Cd(GS)2 complex, about the nature of ligands involved in cadmium chelation by glutathione, and provide insights on the structures of Cd(GS)2 complexes in solution at different pH. We also show that γ-Glu-Cys, the precursor of glutathione, forms a stable complex with cadmium, but biological studies of the first steps of cadmium detoxification reveal that this complex does not seem to be relevant for this purpose. |
| Databáze: | OpenAIRE |
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